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Instructions for labor protection when working in clinical diagnostic laboratories

Occupational Safety and Health

Occupational Safety and Health / Standard instructions for labor protection

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Safe Operation

1. General safety requirements

1.1. To work in clinical diagnostic laboratories (hereinafter referred to as the laboratory), persons are allowed who are at least 18 years old, who have received a completed medical education, as well as special instruction, who do not have contraindications in accordance with the order of the Ministry of Health of the Russian Federation No. on electrical safety

1.2. Laboratory staff must undergo a mandatory medical examination upon admission to work and periodically at least once every 12 months.

1.3. All new entrants to work, regardless of their position, must undergo an introductory briefing by an OT engineer. The results of the briefing are recorded in the log of introductory briefing on labor protection. After that, the final registration of the newly arriving employee and his direction to the place of work is carried out.

1.4. Each newly hired to the laboratory must undergo an initial briefing at the workplace. Re-instruction at least 1 time in 6 months. The results of the briefing are recorded in the briefing log at the workplace.

1.5. Upon admission to work and at least once every 1 months, an examination of the knowledge of personnel on labor safety issues must be carried out according to a program approved by the chief physician.

1.6. The laboratory staff is obliged to comply with the internal labor regulations, work and rest schedule.

1.7. When working in the laboratory, exposure to the following is possible. dangerous and harmful factors.

  • increased voltage in the electrical network, the closure of which can occur through the human body;
  • danger of injury from fragments of dishes used in the process of work;
  • increased level of toxic products of poisonous, flammable substances in the air of the working area;
  • increased dust content in the air of the working area;
  • increased tension of the organs of vision;
  • the risk of contamination of personnel in the study of infectious materials.

1.8. The office staff must.

  • be guided in the work by their job description;
  • know how to provide first aid, know the location of the first aid kit;
  • know the rules of fire safety and the location of fire extinguishing equipment.

1.9. The administration of the institution is obliged to uninterruptedly provide the employees of the department with sanitary clothing, overalls, safety shoes, and other PPE.

The office staff is obliged to follow the rules of personal hygiene, the rules for wearing sanitary clothing and footwear, PPE.

1.10. An eyewitness or victim is obliged to immediately notify the head of the office about each accident related to production. The head of the cabinet must provide first aid, notify the heads of the doctor and the OT engineer. To investigate an accident, it is necessary to maintain the workplace environment and the condition of the equipment as it was at the time of the accident, if this does not threaten the life and health of others and does not lead to an accident.

1.11. Persons who violated the OT instructions are subject to disciplinary action, and, if necessary, an extraordinary knowledge test.

2. Safety requirements before starting work

2.1. Before starting work, you must:

  • turn on ventilation 30 minutes before starting work;
  • put on sanitary clothes and shoes, prepare PPE, rubber gloves, if necessary, a respirator, an apron

2.2. The laboratory personnel must check the readiness for operation of the equipment, its grounding, subject it to wet cleaning, report the noticed malfunctions to the head of the office and not start work until they are eliminated.

2.3. Check the operation of electrical appliances, grounding, local lighting, gas burner, ventilation, small-scale mechanization.

3. Safety requirements during work

3.1. Laboratory staff should not rush during work, analyzes should be carried out taking into account safe techniques and working methods.

3.2. When connecting electrical equipment to the network, it is necessary to check the compliance of the voltage of the device indicated in the passport with the mains voltage, as well as the presence of grounding.

3.3. All heating appliances must have a smooth surface, be accessible for easy cleaning and must be installed on heat-insulating materials.

3.4. The laboratory person must monitor the integrity of glassware, equipment and utensils and prevent the use of broken objects in the work.

3.5. A laboratory worker should not lift a load exceeding 7 kg alone.

3.6. Workplaces for conducting urine and feces studies, biochemical, serological and hormonal studies should be equipped with mechanically driven fume hoods.

3.7. The fume cupboard doors should be kept as closed as possible during operation. They can only be opened during instrument maintenance and installation. Raised sashes must be firmly fixed with devices that prevent unexpected fall of these sashes.

3.8. At the entrance of the gas network to the laboratory, a common gas valve is installed, which is closed at the end of the working day. Gas burners on work tables and fume hoods must have taps. Gas burners must be kept clean and tidy, for which they should be periodically disassembled and cleaned. In the event of a temporary interruption in the gas supply, it is necessary to close the gas valves for all appliances.

3.9. When operating centrifuges, the following requirements must be observed:

  • when loading the centrifuge with glasses or test tubes, follow the rules of strict pair balancing;
  • before connecting the centrifuge to the electrical network, it is necessary to check whether the cover is well screwed to the body;
  • turn on the centrifuge in the electrical network smoothly with the help of a rheostat, after turning it off, it is necessary to allow the rotor to stop, it is forbidden to brake the rotor by hand;
  • after work, the centrifuge must be inspected and wiped.

3.10. When operating the thermostat, the following requirements must be observed:

  • it is forbidden to put flammable substances in the thermostat;
  • Clean the thermostat only after disconnecting from the mains.

3.11. To prevent fatigue and damage to vision during microscopy and the use of other optical devices, it is necessary to provide illumination of the field of view provided for a given microscope or device, do not close the idle eye, work alternately with one or the other eye, and take breaks in work when vision is fatigued.

3.12. Before each analytical balance it is necessary to have lamps.

3.13. Cylinders with compressed gases must have safety caps. Cylinders should not be placed in places illuminated by direct sunlight, they should not be near heaters, heaters and come into contact with electrical wires. The distance from radiators and other heating appliances to cylinders must be at least 1 m, and from other sources of heat with an open fire at least 5 m. Cylinders must be carefully fixed in a vertical position.

3.14. The release of gas from the cylinder must be carried out through a reducer designed exclusively for this gas. The valve opens slowly. It is impossible to be in front of the reducer in the direction of the axis of the valve fitting, while opening the cylinder valve. When emptying the cylinder, an excess pressure of at least 0,5 kg per cmXNUMX must remain in it. It is forbidden to use cylinders that do not have inscriptions and colors established for this gas.

3.15. When unpacking the infectious material sent to the laboratory for research, the jars and test tubes containing the materials are wiped with a disinfectant solution and placed on metal trays.

3.16. When conducting bacteriological studies with infectious materials, the following rules must be observed:

  • before work, carefully check the integrity of glassware, the patency of needles and pistons for syringes;
  • do not touch the test material and the water condensate in the inoculated dishes with your hands. Work with infectious material should be carried out using tools (tweezers, loops);
  • sowing in test tubes and Petri dishes should be carried out near a burning burner with burning of the loop, spatula and edges of the test tube;
  • transfusion of infectious fluids from a vessel into a vessel over the edge is not allowed;
  • when inoculating infectious material on test tubes, cups, flasks, vials and other utensils, inscriptions are made indicating the name of the material, analysis number and date of inoculation;
  • it is forbidden to leave unfixed smears, Petri dishes, test tubes and other utensils with infectious material on the tables;
  • in the room intended for the processing and sowing of infectious material, it is prohibited to carry out other types of work.

3.17. In the laboratory premises it is prohibited:

  • leave lit burners and other heating devices unattended;
  • light a fire and turn on the current if the laboratory smells of gas;
  • pour fuel into a burning spirit lamp, use a spirit lamp that does not have a metal tube;
  • carry out work related to distillation, grinding of harmful substances in case of faulty ventilation;
  • when working in a fume hood, keep your head under the draft;
  • taste and inhale unknown substances;
  • tilt your head over a vessel in which some liquid boils;
  • store stocks of poisonous and explosive substances;
  • store and use unlabeled reagents;
  • store and eat food, as well as smoke;
  • perform work not related to the task;
  • block up the aisles.

4. Safety requirements in emergency situations

4.1. In the event of an accident, office staff should:

  • notify the head of the laboratory;
  • disconnect the equipment from the mains;
  • in case of a short circuit, breakage in power supply systems, turn off the main switch in the room;

4.2. At the slightest sign of gas leakage and faulty burners, work should be stopped until the gas leak is eliminated and the burners are replaced.

4.3. In case of spillage of acids, alkalis, laboratory personnel must take the necessary measures to eliminate the consequences.

4.4. In case of electric shock or other injuries, act according to the instructions for providing first aid to victims of electric current.

4.5. In the event of a power outage, a short circuit, a break in the power supply systems, or if there is a smell of burning, the personnel must turn off the electrical equipment and call an electrician.

4.6. In the event of a fire, evacuate the patient, call the fire brigade, and before the arrival and meeting of the fire brigade, extinguish the fire with primary fire extinguishing equipment.

4.7. In case of breakdowns in the communication systems of water supply, sewerage, heating and ventilation, which impede the performance of technological operations, stop work until the accident is eliminated.

5. Labor protection requirements at the end of work

5.1. Upon completion of work with infectious material used glass slides, pipettes, spatulas are immersed for one day in jars with a disinfectant solution, then washed and boiled.

5.2. Dishes with used nutrient media, feces, urine taken from infectious patients are collected in tanks and disinfected by steam sterilization.

5.3. The laboratory staff is obliged to put the workplace in order, turn off the ventilation, remove sanitary clothing and put it in the designated place. Hand over gauze bandages for sanitization. Dispose of disposable respirators for recycling.

5.4. When cleaning the premises at the end of the working day, the floors are washed with a disinfectant solution.

5.5. Wet cleaning of all premises is carried out daily. Periodically, at least once a month, a complete cleaning should be carried out with washing of walls, floors, doors, window sills, and the inside of windows.

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