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Real-time sequencing

28.07.2016

Scientists from the University of Nottingham (England), led by Dr. Matt Loose (Matt Loose), together with Oxford Nanopore Technologies, have developed a compact device that can decipher the desired sections of DNA "on order", in real time.

The handheld device, called MinION, is based on nanopore sequencing. Its essence is as follows: long organic molecules (for example, DNA) pass through the pores in the membrane, to which an electrical voltage is applied. In this case, the ion current through the pore changes depending on which part of the molecule passes through the pore - for example, which nucleotide. By registering these changes (special software does this in MinION), it is possible to decipher (sequence) the sequence of nucleotides in DNA.

The breakthrough achievements of British scientists is that the device they created is, firstly, compact and (relatively) easy to use, and secondly, it works in real time. The latter was achieved by accelerating the movement and processing of information inside the device. Thanks to this, it became possible to identify a section of DNA even before it completely passes through the pore. After identification, the system compares the section being processed with the one that needs to be decrypted, and gives the command to continue doing this or skip this section and immediately go to the next one (with which the same procedure will be repeated). This technology is called "Read Until".

There is little doubt that MinION can significantly accelerate research in various fields of biology and medicine. "Applying this approach to a wide range of problems, from pathogen detection to sequencing of target regions of the human genome, is a matter of the near future," said Dr. Luz.

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