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Egg Recovery

11.11.2015

Harvard hosted another Ignobel Prize ceremony. The winners were ten scientific teams, whose representatives during the ceremony listened to the mini-opera in three acts, delivered the required 60-second speech and received ten trillion dollars (Zimbabwean). In particular, the prize in chemistry was awarded to researchers from the University of California at Irvine and their Australian colleagues, who "partially restored a boiled egg."

It would seem, who needs such a restorative operation? Let's discuss. A boiled egg differs from a raw one in that its proteins have lost their structure due to heating, that is, they have denatured. Approximately in this way, losing structure, large proteins also suffer when they are removed from cells. A protein that has lost its structure also loses its inherent properties, primarily healing ones. In addition, large proteins tend to entangle and form insoluble aggregates. Therefore, to extract those proteins that bacteria and yeast synthesized from the matrices of the introduced genes is a separate task for biotechnologists, and in complex cases it is necessary to use more capricious cells of insects and mammals. All this leads to a rise in the cost of biotechnological products, primarily medicines. The authors of the work wanted to reduce the cost of this technology.

They really took the protein of a chicken egg, boiled it, then dissolved it in urea and began to work with this solution. Namely: poured into a test tube and spun it in a centrifuge at high speed. The liquid formed a thin film on the test tube wall, and strong shear stresses arose inside it. They turned out to be very useful - in just two and a half minutes, the structure of one of the proteins, lysozyme, was restored. Further - more: it was possible to restore the structure of caveolin synthesized by the bacterium, a membrane protein of vertebrates; extraction accelerated a hundred times, and its properties improved significantly. The larger catalytic domain of the protein kinase had to be tinkered with, but not without success.

From this work, a successful industry may well be born. Not without reason, in the biography of some researchers, the Nobel Prize followed the Ignobel Prize ...

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