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Heat controlled microbe

25.04.2012

Scientists at the University of Georgia have created a temperature-controlled microbe. The new microorganism could be an efficient means of producing complex chemicals from simple starting materials.

Initially, scientists took the microbe Pyrococcus furiosus, which lives in hot marine sediments and actively reproduces at temperatures around 100 degrees Celsius. This microorganism is an archaea, a single-celled prokaryote that is similar to a bacterium, but has significant differences at the molecular level and is able to perform processes that bacteria cannot.

Nowadays, many manufacturing processes depend on microorganisms. However, the production activity of bacteria is very difficult to control, especially to switch them from one activity to another.

American scientists have found a way to control heat-loving microbes using a temperature switch. Simply put, at high temperatures, the microbe produces one product, and at low temperatures, another or not at all. This greatly simplifies the use of microorganisms as miniature plants for the production of useful substances such as biofuels. Also, for the first time, scientists have managed to make changes to the genome of a hyperthermophilic bacterium, which opens up new opportunities in the creation of genetically modified microorganisms with unique functions.

Like other hyperthermophilic bacteria, Pyrococcus furiosus can function at high temperatures, which is very useful in many industrial processes in the chemical industry. However, not all substances can be produced under such conditions - some enzymes can only work at low temperatures.

To solve this problem, scientists took a specific gene from the bacterium Caldicellulosiruptor bescii, which lives in cooler conditions (72 degrees Celsius), and placed it in the genome of Pyrococcus furiosus. As a result, at high temperatures, Pyrococcus furiosus functions as usual, and when the temperature drops, it begins to produce another, unusual product, lactate dehydrogenase.

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