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Living synthetic cells created

24.09.2022

Researchers at the University of Bristol in the UK have taken a big step forward in the field of synthetic biology. Scientists have developed a system that performs several key functions of a living cell, including energy generation and gene expression.

Scientists have previously focused on getting artificial cells to perform a single function, such as gene expression, enzyme catalysis, or ribozyme activity. If scientists unlock the secret to individually creating and programming artificial cells that can more closely mimic life, it could open up a myriad of possibilities in everything from manufacturing to medicine.

While some engineers are focusing on redesigning the cells themselves, others are looking for ways to cut existing cells down to fragments that can then be redesigned into something relatively new.

To accomplish this latest bottom-up bioengineering feat, the researchers used two bacterial colonies, Escherichia coli and Pseudomonas aeruginosa. These two bacteria were mixed with empty microdroplets in a viscous liquid. One population was captured inside the drops, and the other was captured on the surface of the drops.

The scientists then cut open the membranes of the bacteria by immersing the colonies in lysozyme (an enzyme) and melittin (a polypeptide derived from bee venom). The bacteria spit out their contents, which were captured by the droplets to create membrane-covered protocells.

Scientists have demonstrated that cells are capable of complex processing, such as the production of the energy storage molecule ATP through glycolysis, and the transcription and translation of genes.

"Our approach to the assembly of living material makes it possible to create symbiotic living / synthetic cell constructs from the bottom up. For example, using engineered bacteria, it will be possible to produce complex modules for development in the diagnostic and therapeutic areas of synthetic biology, as well as in biomanufacturing and biotechnology in general," - noted the first author, chemist Can Xu.

In the future, this kind of synthetic cell technology could be used to improve the production of ethanol for the biofuel and food industries. In addition, such artificial cells can be programmed for photosynthesis, like purple bacteria, or for generating energy from chemicals, like sulfate-reducing bacteria do.

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Random news from the Archive

Clay XNUMXD Printing 27.05.2009

American materials scientists managed to make XNUMXD printing cheaper.

Three-dimensional printing, that is, the production of three-dimensional objects from a computer model using a special printer, is a very interesting technology. Today it is used not only for designing machine parts or making individual prostheses: sculptors and museum workers have also begun to be interested in the possibility of creating small editions of works of art.

As a result, there are more and more students who come to learn how to work with such equipment. This means that the need for consumables is increasing, and they are expensive.

“We are now spending up to $4000 a quarter to buy branded clay for such a printer. This is too much for our budget. In fifteen years of operation, we have spent so much money on consumables that the initial cost of a printer - $ 20 - seems now just ridiculous " says Mark Gunter of the University of Washington.

To keep costs down, he and his colleagues created clay-based compositions for artists. Store-bought sugar and maltodextrin were added to it. As a result, the cost of consumables has decreased by fifty times, and working with them is no worse than with branded ones.

Well, if the printer still breaks down, scientists will repair it with their own hands - the guarantee will be lost. But cheap material is indispensable for teaching students.

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